Part:BBa_K3468017:Design

PETase Q182I

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. By finding suitable sites around A183, F201, and A240 for mutations, the hydrophobic interaction between the residues is enhanced and the hydrophobic core is created, so Q182I was designed.

Source

Ideonalla sakaiensis